Physico-chemical characterization of bacterial and hemagglutinins from the serum of the mud crab Scylla serrata
نویسندگان
چکیده
A naturally occurring hemagglutinin (HA), with activity against bacteria and yeast cells were detected in the serum of Scylla serrata using mammalian erythrocytes (RBC), various bacteria and yeast as indicator cells. The serum gave highest HA titer with rabbit RBC, tripsinized yeast and Vibrio fluvialis. An analysis of the physico-chemical properties of the HA showed it to be specifically dependent on the presence of Ca for its activity, stable between pH 7 to 9 and showed thermal stability between 10 to 30 °C. Further studies demonstrated that the HA is precipitable by ammonium sulphate and TCA. HAinhibition assays performed with carbohydrates revealed that the serum HA was specific for non-reducing terminal glucose with 1-2 glucosidic linkages. Thus this agglutinin appears to be unique among all the known crustacean agglutinins.
منابع مشابه
The Population dynamics of mud crab (Scylla serrata) in coast of Bandar Khamir (Hormozgan Province)
The Sampling one-year period from October 92 to October 93 on a monthly basis was conducted using gillnets and baited traps. 70% of gillnet catches during flood tide consisted of adults. Sub-adults and juveniles were 28 % and 4.5%, respectively. Similarly, during ebb tide consisted of 51.7%, 24.2%, and 24.1%, respectively. It suggests that gillnets are not sized selective gear. In contrast, bai...
متن کاملNon-indigenous giant mud crab, Scylla serrata (Forskål, 1775) (Crustacea: Brachyura: Portunidae) in Malaysian coastal waters: a call for caution
Introduction of non-indigenous species into a well-established ecosystem can be detrimental, resulting in both ecological and economical damage. Two specimens of giant mud crab, Scylla serrata were found for the first time in two geographically distinct mud crab landing sites (Matang Mangrove Forest Reserve, Perak and Kota Marudu Mangrove Forest, Sabah) in Malaysia. Their identities were confir...
متن کاملThe embryonic development of orange mud crab, Scylla olivacea (Herbst, 1796) held in the captivity
This study attempts to observe and record early embryonic developments of the orange mud crab, Scylla olivacea. The period taken by the eggs to hatch was 8 days and the colour of the eggs gradually changed from yellow to brown, gray and dark gray. During the embryonic development, the developing embryos reached the blastula stage within 24 hours with a mean egg diameter of 329.91 µm ± 6.62. The...
متن کاملA Reo-like virus associated with high mortality rates in cultured mud crab, Scylla serrata, in East China
The discovery of a reo-like virus in cultures of the mud crab Scylla serrata (Forsskål, 1755) is reported here. Since May 2004, in Zhejiang Province, Peoples Republic of China, reo-like virus in mud crabs has reached serious epidemic levels, causing mortality rates exceeding 90%. Beginning in May 2004, moribund crabs from different districts and areas were investigated. By using electron micros...
متن کاملThe embryonic development of orange mud crab, Scylla olivacea (Herbst, 1796) held in the captivity
This study attempts to observe and record early embryonic developments of the orange mud crab, Scylla olivacea. The period taken by the eggs to hatch was 8 days and the colour of the eggs gradually changed from yellow to brown, gray and dark gray. During the embryonic development, the developing embryos reached the blastula stage within 24 hours with a mean egg diameter of 329.91 µm ± 6.62. The...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2010